Renal Genomics Core - Duke O'Brien Center for Kidney Research

The objective of this Core is to facilitate studies aimed at understanding the genetic mechanisms of CKD, CVD, and hypertension individually, and exploring their shared pathways of genetic risk.

Based on recent advances in genomic science, there are exciting and myriad opportunities for human genetic discovery. However, individual investigators are often unable to take advantage of these advances due to lack of access to the required infrastructure and resources. This includes not only molecular genetic tools but also access to expertise in study design, genetic epidemiology and analytical approaches that are critical for successful discovery.

The Renal Genomics Core (RGC) capitalizes on superb existing institutional resources at the Duke Center for Human Genetics (CHG) and the Duke Center for Human Genome Variation (CHGV) to reduce the barriers to these critical core services for individual investigators who are interested in exploring the genetic basis of problems in CKD, CVD, and hypertension.

Through our DOCK Renal Genomics Core, we expect to facilitate the identification of genetic variants that are associated with risk for CKD, CAD and hypertension, individually or in combination.

  • Pre-study consultation service as an entry point for investigators interested in carrying out cardio-renal genetic studies. The purpose of this service is to provide assistance for study design, development, implementation and coordination of study protocols with investigators, including power analyses, human subjects IRB preparation, programming and informatics.
  • Provide a standardized service for DNA isolation and storage. This will include capabilities for banking tissue samples and DNA, performing DNA extraction with removal of serum and plasma, and allocation of samples.
  • Provide assistance in carrying out molecular genetic studies. This will consist of providing access to technical capabilities and expertise for genome-wide association/linkage studies, as well as whole genome/exome sequencing. The Core will also carry out follow up targeted Sanger sequencing for gene identification. Along with the technical services, we will also provide raw results data management, quality control, statistical and bioinformatic analysis incorporating the latest advances in statistical and genetic methodology. The Renal Genomics Core will provide our users with comprehensive genomics services that can be effectively utilized regardless of their level of experience, taking advantage of the latest technical advances. Access to these powerful tools will promote advances in understanding the genetic architecture of the complex interactions of the kidney in cardiovascular disease and hypertension.